Omics · study · 2026
Transcriptional Regulation by NFATc1 and NFATc2 in chronically activated CD8+ T cells
Listed in NCBI GEO
Both chronic viral infections and progressing tumors result in dysfunctional CD8+T cells in a process called exhaustion.
Description
Such TEX cells exhibit a reduced proliferative capacity, reduced cytokine expression and target cell killing. They are characterized by increased expression of co-inhibitory receptors as PD-1, Tim-3, Lag-3 and TIGIT, and resistance against immune checkpoint therapies (ICT).
These cells develop from so called precursors of exhaustion (TPEX) that express high levels of TCF1 and Ly108 and still respond to ICT. The NFAT factors NFATc1 and NFATc2 play a crucial role in exhaustion of CD8+ T cells and, thereby, in suppression of tumor formation by immune cells. NFATc1 is the most prominent NFAT factor in nuclei of activated T cells and the inducible isoform NFATc1/aA, is strongly expressed in chronically induced CD8+ T cells.
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The inducible expression of NFATc1/aA is controlled by the distal enhancer E2 and conditional ablation of E2 abolish this induction. We compared here the transcriptome of in vitro chronically stimulated CD8+ T cells with different NFAT deficiencies. While the ablation of NFATc2 resulted in reduced expression of exhaustion markers Tim3 and Tigit and increased expression of Tcf7 and Slamf6, only minor effects on exhaustion markers were detected upon ablation of inducible NFAT1/aA or entire NFATc1.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE253nnn/GSE253492 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE253492 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1065893 ncbi.nlm.nih.gov/bioproject/PRJNA1065893 ↗
project · from NCBI GEO
- PubMed 42568576 pubmed.ncbi.nlm.nih.gov/42568576 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE253492 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |