Data · dataset · 2026
Supplementary Figure 6 from The H63D Polymorphism in the <i>HFE</i> Gene Promotes Aggressive Disease Phenotypes in Pancreatic Ductal Adenocarcinoma
Listed in ZivaHub and Deakin Research Online and DMU Figshare — shown once because both records carry DOI 10.1158/1078-0432.34041284
<p>(A) qRT-PCR screening of three independent <i>HFE</i>-targeting siRNAs (siRNA1–3), a pooled siRNA mixture, scramble control, and untreated control in MiaPaCa2 cells (WT for <i>HFE</i> mutation).
Description
Relative <i>HFE</i> transcript levels are shown as ΔCt values normalized to 18S.(B) qRT-PCR screening of the same siRNA panel in PANC-1 cells (H63D carriers). (C) Representative Western blot showing HFE and TWIST1 protein expression in MiaPaCa2, A8184, PANC-1, PANC-1 + <i>HFE</i> siRNA, and PANC-1 + scramble.
GAPDH was used as loading control. (D) Densitometric quantification of HFE protein levels normalized to GAPDH. (E) Relative mRNA expression of EMT-transcription factor genes (CDH1, CDH2, TWIST1, ZEB1, ZEB2) in wild-type PDAC cell lines (A8184 and MiaPaCa2) following <i>HFE</i> silencing or control treatment.
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(F) Relative mRNA expression of cell-cycle regulators (<i>CCNC, CCND1, CCNE, CCNA, CCNB</i>) in A8184 and MiaPaCa2 cells after <i>HFE</i> knockdown or control treatment. (G) Densitometric quantification of TWIST1 protein levels normalized to GAPDH. (H) Cell-cycle phase distribution of PANC-1 cells (homozygous H63D) under control conditions or following HFE siRNA treatment.
(I) Relative mRNA expression of EMT-related genes (<i>CDH1, SLUG, TWIST, ZEB2, SNAIL</i>) in synchronized PANC-1 cells analyzed in G1 and G2 phases under untreated, scramble, or <i>HFE</i>-silenced conditions. Values are presented as median ±SD. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant.</p>
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Where it is published
- DOI doi.org/10.1158/1078-0432.34041284 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Cancer · Cancer · Cancer · Earth & Environmental Science · Earth & Environmental Science · Earth & Environmental Science · Life Sciences · Life Sciences · Life Sciences · Medicine & Health · Medicine & Health · Medicine & Health
- Inferred from text
- Disease 75% · Oncology and carcinogenesis 74%
Provenance · 3 source records, 18 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34041284 | 11 d ago | JSON v1 |
| Deakin Research Online | oai:figshare.com:article/34041284 | 11 d ago | JSON v1 |
| DMU Figshare | oai:figshare.com:article/34041284 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | mapping · dro deakin edu au | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:cancer | mapping · figshare dmu ac uk | vocabulary-mapper@1.0.0 | keywords['Cancer'] |
| concepts[disease].local:disease:disease | enrichment · zivahub uct ac za | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3211 | enrichment · zivahub uct ac za | taxonomy-embedding@1.1.0 | title+keywords+description (74%) |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · dro deakin edu au | connector:dro_deakin_edu_au@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare dmu ac uk | connector:figshare_dmu_ac_uk@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license_text | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |