Omics · study · 2026
RNA-seq analysis of U251 cells following tRF-3021a knockdown at 8 and 48 hours
Listed in NCBI GEO
tRNA-derived fragments (tRFs) are relatively recently discovered small RNAs implicated in gene-regulatory processes in diverse biological contexts, but there have been few reports of clear phenotypic roles for these small RNAs in cancer progression.
Description
High expression of the 3' tRFs tRF-3009a, tRF-3021a, and tRF-3030a is associated with poor overall survival in low-grade glioma. In glioblastoma cells, these tRFs enhance invasion and migration, while tRF-3021a is uniquely required for cell proliferation, suppression of apoptosis, and maintenance of global protein synthesis.
This RNA-seq study profiles transcriptomic changes following tRF-3021a knockdown in U251 cells at early and late time points (8 hours and 48 hours)
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE335nnn/GSE335248 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE335248 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1477797 ncbi.nlm.nih.gov/bioproject/PRJNA1477797 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 65%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE335248 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |