Omics · study · 2026
Lgr4 maintains Lgr5+ crypt base columnar cell identity required for intestinal homeostasis and regeneration [RNA-Seq]
Listed in NCBI GEO
The intestinal epithelium is amongst the most rapidly self-renewing epithelia in mammals.
Description
Wnt signalling is known to be essential for intestinal homeostasis and regeneration. However, the underlying cellular hierarchy remains debated.
Recent models have challenged dogma with a LGR4/FGBFBP1+ isthmus cell being upstream of the Lgr5⁺ crypt base columnar (CBC) cells. Importantly the precise functional roles of Lgr4 and Lgr5 not fully understood. Here we show that Lgr4 is broadly expressed within the intestinal epithelial cells (including in Lgr5+ CBCs) and is required directly for Lgr5+ CBCs, Paneth cells, long term crypt survival and regeneration.
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Lgr5 loss expands CBC markers and Paneth cells at the expense of Goblet cells. Loss of Lgr4 function can be rescued by loss of the E3 ubiquitin ligases ZNFR3/RNF43. Together, these findings establish LGR4 as the principal Wnt-potentiating Lgr receptor and an indispensable, regulator of Lgr5+ CBC cell identity in intestinal homeostasis and regeneration.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE343nnn/GSE343667 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE343667 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1512364 ncbi.nlm.nih.gov/bioproject/PRJNA1512364 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE343667 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |