Identification of genomic features that uniquely impact estrogen receptor alpha binding and its effects on gene expression in endometrial cancer [ATAC-seq]

However, the transcription factors that predict ER activity are found to be cell type-specific, including FOXA1 and GATA3 in T-47D cells, and ETV4 and SOX17 in Ishikawa cells. In addition, the features that predict ER binding and effects on gene expression differ, with transcription at EREs in the absence of estrogen being predictive of regulatory activity. To verify our findings in the Ishikawa cells, we perform a CRISPR knockout screen, which confirms the discovery that SOX17 controls ER activity in endometrial cancer cells.

These results identify important genomic features of ER binding and regulatory activity and how these features differ between endometrial cancer and breast cancer cells.