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Functional Proteomic Analysis of HT-29 Cells Treated with MV-GFP, Triptolide, or Combination Using RPPA

Listed in NCBI GEO

Reverse Phase Protein Array (RPPA) analysis was performed on HT-29 cells to evaluate the effects of vehicle, MV-GFP (MOI 0.5), Triptolide (6.25 nM), and their combination on protein expression.

Description

Protein lysates were collected after 48 hours of treatment. RPPA was performed at the MD Anderson Cancer Center RPPA Core Facility.

Differential protein expression was analyzed using the limma R package (v3.26.9), with p < 0.05 and FDR < 0.2.

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Life Sciences
Provenance · 1 source records, 9 field assertions
SourceKeyLast seenRaw
NCBI GEOGSE31334115 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[disease].local:disease:cancerenrichment · NCBI GEOkeyword-concept-rules@1.0.0title+description (75%)
concepts[field].anzsrc:group:3102enrichment · NCBI GEOtaxonomy-embedding@1.1.0title+keywords+description (71%)
concepts[field].local:field:life-sciencesmapping · NCBI GEOconnector:ncbi_geo@1.0.0
concepts[method].geo_series_type:protein-profiling-by-protein-arraysource · NCBI GEOconnector:ncbi_geo@1.0.0/gdstype
concepts[organism].NCBITaxon:9606source · NCBI GEOconnector:ncbi_geo@1.0.0/taxon
descriptionsource · NCBI GEOconnector:ncbi_geo@1.0.0/summary
publication_datesource · NCBI GEOconnector:ncbi_geo@1.0.0
titlesource · NCBI GEOconnector:ncbi_geo@1.0.0/title