Omics · study · 2026
Investigating the potential of an α7 nicotinic acetylcholine receptor agonist in adult mammalian retinal neurogenesis through Müller glia reprogramming and progenitor cell formation
Listed in NCBI GEO
Purpose: Adult mammalian Müller glia (MG) typically undergo a gliotic response after injury that inhibits neuronal regeneration.
Description
Here, we tested whether the α7 nicotinic acetylcholine receptor agonist PNU-282987 can promote MG proliferation and progenitor formation.
Methods
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Primary retinal pigment epithelium (RPE) cells were treated with 100 nM PNU-282987 or 0.1% DMSO, then co-cultured with primary MG cells. RNA was extracted, sequenced on the Illumina NovaSeq platform, and mapped to the Mus musculus (GRCm38) genome. Differential expression was assessed (|log2FC| ≥ 1, padj ≤ 0.05).
Results: Significantly differentially expressed genes (sDEGs) were identified, including genes associated with progenitor formation, retinal development, and regeneration. Selected targets were validated by RT-qPCR. Conclusion:PNU-282987 treatment induced transcriptomic changes in MG cells consistent with progenitor formation and regenerative/developmental signaling, with more robust effects than those observed in immortalized MG lines.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341401 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341401 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1502561 ncbi.nlm.nih.gov/bioproject/PRJNA1502561 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 69%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE341401 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |