Imaging · study · 2026
Spatial transcriptomics profiling of NDMA-induced liver injury in Mgmt-deficient mice across sex and time
Listed in NCBI GEO
Spatial transcriptomics (10x Genomics Visium) was performed on fresh-frozen liver tissue (left lobe) from Mgmt-/- mice treated with N-nitrosodimethylamine (NDMA) or saline vehicle during the juvenile period.
Description
The study includes male and female mice sacrificed at 10 weeks or 10 months post-birth to characterize spatially resolved transcriptomic changes associated with NDMA-induced liver damage and potential carcinogenesis over time.
Mice carried the RaDR+/+; GPT+/+ genotype enabling detection of recombination and mutation events. Tissue sections were processed using the 10x Visium workflow, H&E stained, imaged, permeabilized for spatially barcoded cDNA synthesis, and sequenced on an Illumina NextSeq 500.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE332nnn/GSE332601 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE332601 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1468680 ncbi.nlm.nih.gov/bioproject/PRJNA1468680 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Genetics 71%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE332601 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3105 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (71%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |