Table · dataset · 2026
A megabarcoding insect reference library highlighting immature stages and dark taxa from contrasting temperate and subtropical ecosystems in China
Listed in figshare
<p dir="ltr">We present the Megabarcoding Insect Reference: Tianjin and Guangxi (MIR-TG), a comprehensively vouchered DNA barcode library addressing two persistent bottlenecks in insect biodiversity science: the identification of immature life stages and the documentation of hyperdiverse “dark taxa”.
Description
Sampling across contrasting temperate and subtropical ecosystems in China, we employed a highly scalable MinION-based megabarcoding workflow.
To maximize data recovery across varying DNA preservation states, a cost-effective HotSHOT extraction was coupled with a dual-amplicon strategy targeting both full-length (658-bp) and mini-barcode (313-bp) <i>COI</i> fragments. From 2,332 individual specimens, we generated 2,174 high-quality sequences. This pipeline yielded a consolidated reference of 593 morphologically defined units across 9 orders and 134 families.</p>
Links
Where it is published
- DOI doi.org/10.6084/m9.figshare.31947231.v1 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
Provenance · 1 source records, 18 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| figshare | oai:figshare.com:article/31947231 | 4 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].anzsrc:field:310401 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Animal systematics and taxonomy'] |
| concepts[field].anzsrc:field:310913 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Invertebrate biology'] |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |