Omics · study · 2026
mRNA sequencing data of hepatic macrophages (Kupffer cells) under endotoxin tolerance, LPS-induced activation, and control conditions
Listed in NCBI GEO
Endotoxin tolerance is an important adaptive response of macrophages following repeated exposure to lipopolysaccharide (LPS), leading to a reduced pro-inflammatory response.
Description
To investigate the transcriptional changes associated with in vitro LPS-induced tolerance in liver-resident macrophages, we isolated primary Kupffer cells from wild-type C57BL/6 mice and subjected them to distinct LPS stimulation protocols in vitro.
Three groups were analyzed: untreated control cells (Ctrl), cells stimulated once with LPS at the second time point (2nd), and cells stimulated with LPS at both the first and second time points (Tol). Total RNA was extracted from each group, and 3'-end mRNA sequencing (QuantSeq 3’ mRNA-Seq) was performed. This dataset provides insight into the transcriptional programs underlying LPS-induced tolerance in Kupffer cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE300nnn/GSE300953 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE300953 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1282733 ncbi.nlm.nih.gov/bioproject/PRJNA1282733 ↗
project · from NCBI GEO
- PubMed 42668090 pubmed.ncbi.nlm.nih.gov/42668090 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 75%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE300953 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |