Data · dataset · 2026
Experimental Protocol and Validation Proposal: In Vitro Evaluation of Dual Fuel Modulation (DFM) and Adaptive Resistance via the Epitranscriptomic DAMER–ALKBH5–ATF4 Axis in Glioblastoma
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<p dir="ltr">This protocol outlines an in vitro experimental strategy for validating Dual Fuel Modulation (DFM) in glioblastoma (GBM) models while simultaneously evaluating candidate adaptive resistance pathways.
Description
The study combines coordinated metabolic perturbation—glycolytic inhibition, System Xc⁻ blockade, polyunsaturated fatty acid enrichment, and partial mitochondrial modulation—with mechanistic assessment of ferroptosis.
Six treatment groups are proposed to distinguish DFM efficacy, ferroptosis specificity, and resistance-axis contributions. The design prioritizes the epitranscriptomic DAMER–ALKBH5–ATF4 axis and BRD4-associated chromatin remodeling as candidate resistance mechanisms. Primary endpoints include cell viability, lipid peroxidation, NADPH/GSH status, ATF4/NRF2 activation, SLC7A11/GPX4 expression, m6A modification, and BRD4 occupancy.
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Statistical evaluation will use the Chou–Talalay combination index to assess synergy. This protocol provides a staged framework for evaluating whether resistance-axis inhibition restores DFM sensitivity and preserves a therapeutic window in non-malignant neural cells.</p>
Links
Where it is published
- DOI doi.org/10.6084/m9.figshare.34018482.v1 ↗
DOI / persistent id · from zivahub uct ac za
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from zivahub uct ac za
Topics
- From keywords
- Cancer cell biology · Earth & Environmental Science · Life Sciences · Medicine & Health
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| ZivaHub | oai:figshare.com:article/34018482 | 6 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].anzsrc:field:321101 | mapping · zivahub uct ac za | vocabulary-mapper@1.0.0 | keywords['Cancer cell biology'] |
| concepts[field].local:field:earth-environmental | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| description | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/description |
| license | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/rights |
| publication_date | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | |
| title | source · zivahub uct ac za | connector:zivahub_uct_ac_za@1.0.0 | /metadata/dc/title |