Profiling of microRNAs in extracellular vesicles derived from mouse normal hepatocyte line AML12

The mechanisms by which AML12 EVs ameliorate liver fibrosis have not been fully investigated and microRNAs are among the EV payload components that are delivered to recepient cells to regulate their functionsl properties. In this study, EVs were isolated by differential centrifugation of culture supernatants from AML12 cells that ahd been maintained in serum-free conditions.

The EVs were characterized by Nanosight Tracking Analysis and Western blot. Three different batches of AML12 EVs were used to isolate total RNAs by QIAGEN miRNeasy kit, and approximately 200 ng of RNAs were subjected to small RNA-seq. A total of 224 miRNAs were identified, with miR-122-5p, miR-378a-3p, miR-210-3p, miR-320-3p, and miR-125a-5p ranked as the top five most abundant miRNAs.