Omics · study · 2026
Understanding the transcriptome changes in HeLa cells after inactivation of endosomes/lysosomes
Listed in NCBI GEO
In this study, we optimized an experimental approach to inactivate endo/lysosomal compartment, inducing the formation of new endosomes.
Description
Endo/lysosomes inactivation was executed through DAB- mediated depletion of endosomes/lysosomes in HeLa cells, which previously internalized Horseradish peroxidase (HRP). These cells, 18 hours after inactivation, can regenerate new endosomes capable of HRP uptake.
We further observed the transcriptional response in HeLa cells driven by endo/lysosomes inactivation, discovering that, unlike the control condition, in which HRP was internalized but DAB solution was not added to the cells, a substantial number of endosomes/lysosomes related genes are activated
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE325nnn/GSE325448 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE325448 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1439936 ncbi.nlm.nih.gov/bioproject/PRJNA1439936 ↗
project · from NCBI GEO
- PubMed 42251155 pubmed.ncbi.nlm.nih.gov/42251155 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE325448 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |