RNA-seq of mouse primary chondrocytes under IL-1b and IL_PGG intervention

Mouse primary chondrocytes were isolated from the articular cartilage of mouse knee joints and cultured under corresponding treatment conditions. Extract total RNA and perform paired end RNA seq on the Illumina NovaSeq 6000 platform.

Use HISAT2 to process raw sequencing readings and compare them with GRCh38. The gene expression level was quantified as TPM and differentially expressed genes were identified using DESeq2. This dataset provides comprehensive transcriptomic insights into the molecular mechanisms of chondrocyte biology in the context of disease models.