Nasal cell-derived alveolar organoid-macrophage co-culture

We previously established human lung tissue-derived alveolar organoids (AlvO) as an in vitro model of alveolar epithelium for studying respiratory viruses. However, an advanced model is needed due to limited access to human lung tissues and the absence of immune cell components.

Here, we developed novel protocols to generate physiological and functional alveolar organoids (nsoAlvO) from readily accessible and expandable nasal cell-derived organoids, and to generate alveolar macrophages (monoAM) from peripheral blood monocytes.