Role of MicroRNA155 in Hippocampal Neurogenesis and GABAergic Signaling in Alzheimer’s Disease [MIR155 Knockout RNA Sequencing Data]

However, its role in other neural cell types remains unclear. Expanding on previous studies showing miR155 upregulation in hippocampal neurons of both AD model mice and AD patients, we found that in human induced pluripotent stem cell (hiPSC)-derived neural stem cells (NSCs), MIR155 regulates NSC proliferation, with MIR155 deletion increasing NSC numbers and overexpression downregulating NSC markers.

In cortical neurons, MIR155 deletion is associated with increased neuronal outgrowth and GABAergic interneuron generation. In cortical organoids, MIR155 deletion promotes ventral patterning and increases GABAergic interneuron formation. Deleting miR155 in an AD mouse model induced the expansion of hippocampal NSCs in the sub-granular zone (SGZ) and the hilus, and promoted an increase in the number of hippocampal GABAergic interneurons, highlighting the novel role of miR155 in adult hippocampal neurogenesis and GABAergic interneuron signaling.