Deciphering the STAT3-PXN positive feedback loop in GBM, IDH-wildtype: transcriptional regulation and inhibition of YB-1 ubiquitination

This study investigated the transcriptional regulatory role of YB-1 in GBM through the deployment of mRNA sequencing (RNA-seq) on U87 and U373 glioblastoma cell lines that underwent stable knockdown of YB-1 utilizing YB-1-targeting short hairpin RNA (shRNA), with vector-delivered scrambled shRNA employed as a control group.

High-throughput sequencing elucidated genome-wide transcriptomic alterations resultant from YB-1 depletion, encompassing differentially expressed genes (DEGs) pertinent to critical cellular pathways. Additionally, functional enrichment analysis employing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) underscored YB-1's probable involvement in tumor progression mechanisms. The findings present a comprehensive resource that enhances our understanding of YB-1-driven molecular mechanisms in glioblastoma.