Mapping the niche of breast cancer metastases in lung and liver [scRNA-seq-10x-o26674]

Yet, these vital sites are characterized by highly diverse cellular microenvironments and physiological functions, suggesting that they may influence cancer cells behavior in divergent ways. Unexpectedly, we find that while the liver microenvironment fosters metastasis-boosting properties of its metastatic seeds and favors secondary spread, the lungs impose a drastic roadblock to the same processes.

Using patient data and mouse models of triple negative breast cancer (TNBC) that spontaneously metastasize to visceral organs, coupled with genetic barcode-mediated metastasis tracing, niche labeling technology and single cell analysis of both tumor cells and their direct microenvironment at each site, we characterize this dichotomy and identify differential microenvironmental factors that contribute to liver vs lung metastasis biology.