Paternal cardiac injury elicits an inflammatory signal relay to the gonads with intergenerational cardiac effects in vertebrates - Bulk_RNA-seq_F1_heart_ZF dataset

Here, we used the zebrafish and mouse models to explore the intergenerational role of cardiac damage and repair. As early as one week after a cardiac cryolesion, male gonads and gametes activated responses associated with inflammation. In sperm, chromatin accessibility was altered in response to cardiac cryolesion and offspring of cryoinjured zebrafish males, revealing changes in cardiac function and cardiac gene expression.

Induction of systemic sterile inflammation in the paternal generation mimicked a cardiac injury effect in the following generation, while anti-inflammatory treatments in the injured paternal generation partially recovered F1 cardiac features. Chromatin accessibility was also altered in mouse testis after a neonatal injury, and F1 hearts revealed alterations in gene expression associated with the immune response, suggesting a conserved role of sterile inflammation as a vector for intergenerational transmission of cardiac injury.

This submission covers the Bulk_RNA-seq_F1_heart_ZF assay.