cisplatin-damaged gene locus-replicate 1-Blank-R2

We utilized a forward chemical genetics strategy to map 7578 cisplatin-damaged genes (CDGs) with >12 fold-enrichment from A549 human lung cancer cells, of which the highly associated signalling pathways include sperm motility, molecular mechanism of cancer, and protein kinase A signalling. The three most frequently cisplatinated penta-nucleotide motifs and genomic region are CTGGG, CCAGG and GCTGG, and promoter, respectively.

Among the CDGs are 1330 enzyme, 747 transcription regulator and 486 transporter genes. Importantly, cisplatin targeted 306 protein kinase genes, accounting for 59% of putative protein kinase genes in the human genome, and 92 protein phosphatase genes. This indicates that cisplatin genome-wide disturbs protein phosphorylation.