Omics · study · 2026
NOP16 regulates the B cell class switch recombination into IgA
Listed in NCBI GEO
Class switch recombination (CSR) is a DNA recombination process in B cells that generates diverse isotypes, playing a crucial role in humoral immune responses.
Description
However, the regulatory mechanisms underlying CSR remain incompletely understood. In this study, we investigated the role of the nucleolar protein NOP16 in B cell CSR.
Using the mouse B cell lymphoma cell line CH12F3-2A, we induced class switching from IgM to IgA via stimulation with CD40L, IL-4, and TGF-β. Compared to wild-type cells, CH12F3-2A cells with NOP16 knockout via CRISPR-Cas9 exhibited a significant enhancement in IgA class switching. To elucidate the transcriptional differences between wild-type and NOP16-deficient cells during IgA CSR, we performed transcriptome analysis by bulk RNA sequencing (RNA-seq).
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE291nnn/GSE291546 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE291546 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1234127 ncbi.nlm.nih.gov/bioproject/PRJNA1234127 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE291546 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |