IGF2BP3 promotes tumour cells survival in organoid pancreatic adenocarcinoma model

Knocking down IGF2BP3 resulted in significant transcriptional divergence at 24H, 48H and 72H compared to control, most strikingly in the PANC1 cell line. The dominant affect of knockdown was widespread reduction in expression levels of genes functionally enriched for Proliferation, TNFA signalling and Apoptosis.

This highlights IGF2BP3 in these cell lines to be acting as a positve regulator of gene programs broadly associated with progression through the cell cycle and identifies many candidate IGF2BP3-linked genes. A high confidence, consistent motif of genes identified as IGF2BP3-regulated in both cell lines included the genes SLC22A18, GET3 and CCNG1.