Omics · study · 2026
Lineage Tracing Reveals a Common Ancestor of Supraclavicular Brown and Inguinal Beige Adipocytes
Listed in NCBI GEO
Like human scBAT, supraclavicular brown adipose tissue (scBAT) consists of loosely connected thin strips of brown adipocyte islands and is located in the mouse neck.
Description
We traced the origin of scBAT to Mef2c-anterior heart field (AHF)-marked (Mef2c-AHF⁺) cells. To investigate the differentiation potential of these cells, we isolated and immortalized Mef2c-AHF⁺ cells (named Mef2c-AHF-P5 cells) from mouse scBAT.
Using this cell line, we performed RNA sequencing to profile gene expression induced by either standard adipogenic induction medium (IM) or a combination of Bmp7 and Rosiglitazone. Our results indicate that IM and the Bmp7-Rosiglitazone (BR) combination induce distinct gene expression profiles during the early differentiation of Mef2c-AHF⁺ cells.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305221 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305221 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1304857 ncbi.nlm.nih.gov/bioproject/PRJNA1304857 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Heart 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE305221 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[anatomy].local:anatomy:heart | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |