Transcriptomic profiling of PRMT2 wild-type and PRMT2 knockout KG-1a acute myeloid leukemia cells

To define the transcriptional consequences of PRMT2 loss, we performed RNA sequencing of KG-1a AML cells comparing PRMT2 wild-type cells with independent CRISPR/Cas9-generated PRMT2 knockout clones. Differential expression analysis identified a common set of genes downregulated upon PRMT2 deletion.

Gene ontology and gene set enrichment analyses showed that PRMT2 loss reduced transcriptional programs associated with leukocyte activation, cell adhesion, migration, and leukocyte cell-cell adhesion. These data support a role for PRMT2 in maintaining adhesion-associated transcriptional programs in AML cells and provide a transcriptomic resource linking PRMT2-dependent signaling to leukemic cell interaction with the bone marrow microenvironment.