Supporting data for "Functional Characterization and Specificity Investigation of anti-LANCL1 antibodies for treating Hepatocellular Carcinoma"

In vivo data from an orthotopic mouse model treated with 19G4 antibody are included, along with western blot analyses of key signaling pathways affected by antibody treatment. For the humanization study, the dataset contains surface plasmon resonance affinity data for nine humanized antibody variants and a chimeric antibody, as well as sphere formation assays and flow cytometry-based binding and specificity analyses using LANCL1-knockdown cells.

Finally, the dataset includes proteomic and structural analyses identifying potential LANCL1 binding partners and off-target candidates, with enrichment calculations, Venn diagram comparisons, TM-score distributions, and three-dimensional structural superpositions of LANCL1 with non-specific proteins. All raw data, processed data, statistical analyses, and visualization files are organized in clearly labeled folders, with detailed calculation procedures provided in accompanying Excel files and graphical summaries available in Prism files.</p>