Omics · study · 2026
Effects of overexpression on miRNA isoforms and the targetome
Listed in NCBI GEO
Translational inhibition or degradation of messenger RNAs by microRNAs represent core regulatory mechanisms with a high grade of complexity proposed by experimental and computational approaches.
Description
One proposed important factor is isoforms of mature microRNAs (isomirs), adding numerous potential targets and functions. For a set of miRNAs (mir-34a, mir-129, mir-873, mir-133b, mir-7), we characterized the impact of their overexpression in cell culture models (HeLa, HEK293T, LUHMES, SH-SY5Y) on the detection of isomiRs.
We observed an astonishing heterogeneity of isomiRs between cell lines, emphasizing a significantly increased isomiR repertoire following artificial overexpression with up to 832 different isoforms per miRNA. Performing a dilution and a time series, we validated a significantly increased isomiR repertoire after 8 hours and for precursor amounts of 0.5 µg. Proteomic analysis further argued for a significant influence of the isomiR repertoire depending on the miRNA amount.
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While 7mer-m8 sites revealed the highest reduction effect in general, we report an increased frequency but lower amplitude of repression with an increasing number of miR-7-5p isoforms. Our data call attention to interpreting the proposed impact of isomiRs on the targetome, especially using overexpression systems.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE213nnn/GSE213724 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE213724 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA882115 ncbi.nlm.nih.gov/bioproject/PRJNA882115 ↗
project · from NCBI GEO
Topics
- Stated by source
- Homo sapiens · Non-coding RNA profiling by high throughput sequencing
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE213724 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:non-coding-rna-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |