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Data · dataset · 2023

TwinNetworkDataset032

Listed in RADAR and RADAR4Memory — shown once because both records carry DOI 10.48606/90

The data comprises bright-field time-lapse images of zebrafish embryos acquired in multiple batches within multi-well plates using an Acquifer Imaging Machine.

Description

Embryos from incrosses of Tg(sebox:EGFP) and incrosses of TE zebrafish strains were used for imaging. Small-molecule signaling pathway modulator LDN-193189 was added to well A2 with a final concentration of 3.5 µM and to well A3 with a final concentration of 4 µM. Small-molecule signaling pathway modulator SB-505124 was added to wells B1-B3 with final concentrations of 4 µM in well B1, 5 µM in well B2, and 6 µM in well B3.

Well A1 did not receive small-molecule signaling pathway modulator treatment. Imaging started with embryos between 2-3 hpf and ended with embryos between 27-28 hpf. Images of embryos were acquired at 180 acquisition time points with a time interval of 501 s between each acquisition time point.

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Individual embryo segments were identified and extracted using a trained neural network for object detection. Within this experiment folder, data are organized by microscope position and embryo number.

Links

Where it is published

Catalogue records · 1

Topics

Inferred from text
Image 65% · Imaging 75%
Provenance · 2 source records, 19 field assertions
SourceKeyLast seenRaw
RADAR10.48606/9010 d agoJSON v1
RADAR4Memory10.48606/9010 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · radar service eu deconnector:radar_service_eu_de@1.0.0
concepts[field].anzsrc:field:461103mapping · radar4memory radar service euvocabulary-mapper@1.0.0keywords['deep learning']
concepts[field].anzsrc:field:461103mapping · radar service eu devocabulary-mapper@1.0.0keywords['deep learning']
concepts[field].anzsrc:group:3102mapping · radar4memory radar service euvocabulary-mapper@1.0.0keywords['computational biology']
concepts[field].anzsrc:group:3102mapping · radar service eu devocabulary-mapper@1.0.0keywords['computational biology']
concepts[field].local:field:computer-science-aimapping · radar4memory radar service euconnector:radar4memory_radar_service_eu@1.0.0
concepts[field].local:field:computer-science-aimapping · radar service eu deconnector:radar_service_eu_de@1.0.0
concepts[field].local:field:earth-environmentalmapping · radar service eu deconnector:radar_service_eu_de@1.0.0
concepts[field].local:field:earth-environmentalmapping · radar4memory radar service euconnector:radar4memory_radar_service_eu@1.0.0
concepts[field].local:field:life-sciencesmapping · radar4memory radar service euconnector:radar4memory_radar_service_eu@1.0.0
concepts[field].local:field:life-sciencesmapping · radar service eu deconnector:radar_service_eu_de@1.0.0
concepts[modality].local:modality:imageenrichment · radar service eu dekeyword-concept-rules@1.0.0title+description (65%)
concepts[modality].local:modality:imagingenrichment · radar service eu dekeyword-concept-rules@1.0.0title+description (75%)
concepts[organism].NCBITaxon:7955mapping · radar4memory radar service euvocabulary-mapper@1.0.0keywords['zebrafish']
concepts[organism].NCBITaxon:7955mapping · radar service eu devocabulary-mapper@1.0.0keywords['zebrafish']
descriptionsource · radar service eu deconnector:radar_service_eu_de@1.0.0/metadata/dc/description
licensesource · radar service eu deconnector:radar_service_eu_de@1.0.0/metadata/dc/rights
publication_datesource · radar service eu deconnector:radar_service_eu_de@1.0.0
titlesource · radar service eu deconnector:radar_service_eu_de@1.0.0/metadata/dc/title