The RNA Binding Protein ZFP36L2 Displays Tissue-Selective mRNA Targeting in Mice [eCLIP-seq]

We observed only one common up regulated gene, Apol11b, among these six different tissues. However, we do observe common trends, specifically an enrichment in protein coding genes in the up regulated genes, consistent with these RBP primarily targeting genes on their 3’ UTRs. Interestingly, we observed a significant increase in the proportion of IG (immunoglobulin) genes being up regulated.

We further performed eCLIP (Enhanced Cross-Linking&ImmunoPreciptation) on a mouse cell line, MLTC-1 cells, to identify direct binding sites of ZFP36L2. AU-Rich Element score (AREscore) analysis revealed enrichment in both up regulated genes and eCLIP peaks, although some differences were observed in flanking residue composition. Our findings provide new insights into the intricate regulatory network orchestrated by ZFP36L2, opening avenues for exploring its potential roles in different tissues.