Table · dataset · 2026
Data_Sheet_1_Biochemical and genetic studies define the functions of methylthiotransferases in methanogenic and methanotrophic archaea.PDF
Listed in figshare
<p>Methylthiotransferases (MTTases) are radical S-adenosylmethionine (SAM) enzymes that catalyze the addition of a methylthio (-SCH<sub>3</sub>) group to an unreactive carbon center.
Description
These enzymes are responsible for the production of 2-methylthioadenosine (ms<sup>2</sup>A) derivatives found at position A37 of select tRNAs in all domains of life. Additionally, some bacteria contain the RimO MTTase that catalyzes the methylthiolation of the S12 ribosomal protein.
Although the functions of MTTases in bacteria and eukaryotes have been established via detailed genetic and biochemical studies, MTTases from the archaeal domain of life are understudied and the substrate specificity determinants of MTTases remain unclear. Here, we report the in vitro enzymatic activities of an MTTase (C4B56_05415) from a thermophilic Ca. Methanophagales anaerobic methanotroph (ANME) as well as the MTTase from a hyperthermophilic methanogen – MJ0867 from Methanocaldococcus jannaschii.
Read the rest (2 more)
Both enzymes catalyze the methylthiolation of N<sup>6</sup>-threonylcarbamoyladenosine (t<sup>6</sup>A) and N<sup>6</sup>-hydroxynorvalylcarbamoyladenosine (hn<sup>6</sup>A) residues to produce 2-methylthio-N<sup>6</sup>-threonylcarbamoyladenosine (ms<sup>2</sup>t<sup>6</sup>A) and 2-methylthio-N<sup>6</sup>-hydroxynorvalylcarbamoyladenosine (ms<sup>2</sup>hn<sup>6</sup>A), respectively. To further assess the function of archaeal MTTases, we analyzed select tRNA modifications in a model methanogen – Methanosarcina acetivorans – and generated a deletion of the MTTase-encoding gene (MA1153).
We found that M. acetivorans produces ms<sup>2</sup>hn<sup>6</sup>A in exponential phase of growth, but does not produce ms<sup>2</sup>t<sup>6</sup>A in detectable amounts. Upon deletion of MA1153, the ms<sup>2</sup>A modification was absent, thus confirming the function of MtaB-family MTTases in generating ms<sup>2</sup>hn<sup>6</sup>A modified nucleosides in select tRNAs.</p>
Links
Where it is published
- DOI doi.org/10.3389/fmicb.2023.1304671.s001 ↗
DOI / persistent id · from figshare com
Catalogue records · 1
- OAI-PMH record api.figshare.com/v2/oai?verb=GetRecord&metadataPrefix=oai_dc&identifier=oai%3Af… ↗
metadata API · from figshare com
Topics
- From keywords
- Astronomy & Astrophysics · Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Humanities · Life Sciences · Medicine & Health · Microbial ecology · Microbial genetics · Microbiology · Mycology · Ocean & Atmospheric Science · Social Science
Provenance · 1 source records, 20 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| figshare | oai:figshare.com:article/33899683 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].anzsrc:field:310703 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbial Ecology'] |
| concepts[field].anzsrc:field:310704 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbial Genetics'] |
| concepts[field].anzsrc:field:310705 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Mycology'] |
| concepts[field].anzsrc:group:3107 | mapping · figshare com | vocabulary-mapper@1.0.0 | keywords['Microbiology'] |
| concepts[field].local:field:astronomy | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:chemistry | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:computer-science-ai | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:earth-environmental | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:economics-finance | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:engineering | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:humanities | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:medicine-health | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:ocean-atmospheric | mapping · figshare com | connector:figshare_com@1.0.0 | |
| concepts[field].local:field:social-science | mapping · figshare com | connector:figshare_com@1.0.0 | |
| description | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/description |
| license | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/rights |
| publication_date | source · figshare com | connector:figshare_com@1.0.0 | |
| title | source · figshare com | connector:figshare_com@1.0.0 | /metadata/dc/title |