Excel · study · 2026
Transcriptional landscape of mouse adipose tissue transfected with LHX8 siRNA
Listed in NCBI GEO
We have studied the role of LHX8 (Lim Homeobox 8) in adipocyte development.
Description
Mouse 3T3-L1 adipcoytes were used for our analyses. Cells were cultured in high glucose DMEM, supplemented with 10% FCS, 1% glutamine and 1% penicillin/streptomycin.
Cells were grown to reach 70-80% confluence, and transfected with a siRNA silencer to decrease Lhx8 mRNA expression. Turbofectamine was used for cell transfection. Total RNA was isolated 48h post-transfection and used for subsequent NGS analysis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE329nnn/GSE329222 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE329222 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1458173 ncbi.nlm.nih.gov/bioproject/PRJNA1458173 ↗
project · from NCBI GEO
- PubMed 42274594 pubmed.ncbi.nlm.nih.gov/42274594 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE329222 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |