m6A modification landscapes of human metapneumovirus, mumps virus, and host A549 cell RNAs revealed by single-base-resolution GLORI-seq [A549 mRNA]

We infected human lung adenocarcinoma A549 cells with multiple nonsegmented negative-sense (NNS) RNA viruses, including Human metapneumovirus (hMPV), Mumps virus (MuV), Vesicular stomatitis virus (VSV), and Sendai virus (SeV), and evaluated the differences among these viruses in activating innate immune signaling pathways in epithelial cells through transcriptomic analyses of infected cells. Furthermore, by integrating the effects of viral infection on the m6A landscape of A549 cell transcripts, we analyzed the correlation between m6A epitranscriptomic regulation and the activation of innate immunity–related transcripts, as well as the role of m6A in viral immune evasion.