Effect of PSNEAT1 knockout on gene expression and ribosome biogenesis in NCI-H1299 cells

PSNEAT1 is a nucleolar microprotein encoded by the NEAT1_1 transcript and has been shown to regulate rDNA transcription and nucleolar function. To investigate the transcriptional consequences of PSNEAT1 depletion, we generated PSNEAT1 knockout NCI-H1299 cells and performed RNA-seq analysis compared with control cells.

Loss of PSNEAT1 resulted in broad gene expression changes associated with ribosome biogenesis, rRNA processing, nucleolar function, and cell growth-related pathways. These data provide a transcriptomic framework for understanding how PSNEAT1 contributes to nucleolar homeostasis and supports rDNA transcription-dependent tumor cell proliferation.