Omics · study · 2026
FTO-Mediated m6A Demethylation Regulates PGC-1α-Dependent Mitochondrial Biogenesis to Attenuate Aluminum-Induced Neuronal Senescence [RNA-Seq]
Listed in NCBI GEO
This study employs integrated MeRIP-Seq and RNA-Seq to investigate how aluminum (Al) exposure drives neuronal senescence via m6A methylation dynamics.
Description
Triplicate samples of mouse hippocampal neuronal cells (HT22) were analyzed under control conditions and following treatment with aluminum maltolate (Al(mal)₃; 60, 120, 240 μM). Total RNA underwent fragmentation and m6A-specific immunoprecipitation (MeRIP) using m6A antibodies and Dynabeads.
Libraries were prepared with the Illumina TruSeq Stranded mRNA Kit and sequenced on the HiSeq X10 platform (paired-end, 150 bp). MeRIP-seq data (aligned to BosGru3.0via HISAT2) revealed Al-induced global hypermethylation (7,068 upregulated m6A peaks, MeTDiff, FDR < 0.05), enriched in coding sequences and annotated to senescence/neurodegeneration pathways (GO/KEGG). RNA-seq identified 6,369 differentially expressed genes (DESeq2, |log₂FC| > 1.5, p < 0.05).
Read the rest (1 more)
Integrative analysis demonstrated coordinated m6A hypermethylation and downregulation of PGC-1αmRNA, linking epitranscriptomic dysregulation to suppressed mitochondrial biogenesis.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE304nnn/GSE304247 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE304247 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1299484 ncbi.nlm.nih.gov/bioproject/PRJNA1299484 ↗
project · from NCBI GEO
- PubMed 42098356 pubmed.ncbi.nlm.nih.gov/42098356 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE304247 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |