Omics · study · 2026
Bulk RNA-seq of U251N orthotopic glioblastoma xenografts in athymic RNU/RNU rats before and after MRI-guided laser interstitial thermal therapy (LITT)
Listed in NCBI GEO
Laser interstitial thermal therapy (LITT) is increasingly used for deep-seated and recurrent glioblastoma, but the transcriptional state of cells that survive the sublethal thermal penumbra and seed recurrence is poorly defined.
Description
We performed bulk RNA-seq on matched primary (pre-LITT) and recurrent (post-LITT) tumors from a U251N orthotopic xenograft in athymic RNU/RNU rats treated with MRI-guided LITT. Reads were sorted into human (graft) and rat (host) streams with xengsort to isolate the human tumor transcriptome from host signal.
Recurrent tumors showed a pronounced translational shutdown and integrated-stress-response activation, with a shift toward the Garofano Mitochondrial molecular subtype, nominating the translation/ISR axis as a candidate target for LITT-adjuvant therapy.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE338nnn/GSE338105 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE338105 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1492510 ncbi.nlm.nih.gov/bioproject/PRJNA1492510 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens · Rattus norvegicus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 65% · RNA sequencing 65%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE338105 | 13 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10116 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |