Effect of extracellular fluid viscosity in macrophages

However, it remains elusive how immune cells, particularly macrophages, respond to a hyperviscous TME. Macrophages likely perceive changes in fluid viscosity as a mechanical stress at the cell membrane, which be transduced into intracellular signals.

To investigate the mechanisms underlying macrophage responses to extracellular fluid viscosity, the whole-transcriptome RNA sequencing on RAW264.7 macrophages cultured in low and high viscosity were performed.