Omics · study · 2026
Type 1 lymphocytes accumulate in the thalamus and produce interferon-gamma to restrict seizure susceptibility after brain injury.
Listed in NCBI GEO
Interferons induce the transcription of a canonical set of interferon-stimulated genes upon binding to their receptor; however, whether these same genes are upregulated within CNS cell types in vivo remained unclear.
Description
To determine what the effects of IFNγ signaling in microglia in vivo, we injected IFNγ (or PBS vehicle control) into the brain of mice and isolated microglia for bulk RNA sequencing 22 hours after injection.
Differential gene expression analysis was then performed to determine which genes were upregulated or downregulated following IFNγ administration. Differentially upregulated genes were then used to construct a microglial IFNγ gene signature that was applied to our other datasets to create a score for IFNγ-responiveness.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE345nnn/GSE345855 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE345855 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1522242 ncbi.nlm.nih.gov/bioproject/PRJNA1522242 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 9 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE345855 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |