Microtubule-RAB5 mutual-influential system screening based on gene-regulatory networks map in Rab5 RNAi eye-degeneration model

Existing studies support the notion that manipulation or mutation of Rab genes could lead to functional changes in neurons. However, whether Rabs gene-manipulation induced Drosophila eye-degeneration remains unknown.

By down-regulating Rab5, but not Rab7, we first constructed a compound eye injury model in Drosophila. As the distribution, content, and even maturation of RAB5-positive endosomes are influenced by cytoskeletal proteins, like actin or tubulin-related proteins, the existence of a bidirectional regulatory relationship between RAB5 and the cytoskeleton is unclear and worth researching. Through complete transcriptome sequencing combined immunofluorescence testing, we confirmed that down-regulation of Rab5 affected the expression of α-Tub84B (alternatively named TubA84B) but not γ-tubulin.