Supplementary file 1_ABRAXAS1-HELQ is a candidate class-matched reference chimeric RNA and contributes to prostate cell motility through MCOLN2.docx

Subcellular localization and biological functions were further evaluated by nuclear-cytoplasmic fractionation, transcriptome analysis, and functional assays.</p>Results<p>Analysis of chimeric RNAs shared between TCGA and CPGEA identified 37 candidate chimeric RNAs without a statistically detectable difference between prostate cancer and matched adjacent tissues. ABRAXAS1-HELQ was detectable in the pooled clinical sample, showed a comparatively consistent expression pattern across nine prostate-derived cell lines, and showed no significant change under selected experimental conditions in three cell lines. siRNA-mediated reduction of ABRAXAS1-HELQ was associated with reduced cell migration, with MCOLN2 identified as a potential downstream mediator.</p>Conclusion<p>ABRAXAS1-HELQ represents a candidate class-matched reference chimeric RNA with relatively consistent expression under the tested experimental conditions and serves as a potential reference molecule for chimeric RNA quantification in prostate-related studies.</p>