Methionine Availability Drives m⁶A-Dependent Regulation of PPARG in Macrophages to Promote Immunosuppression in Glioblastoma

Although tumor metabolism is closely associated with immune suppression, the mechanisms by which elevated methionine availability affects macrophage function remain incompletely understood. In this study, we investigated whether a high-methionine environment alters N6-methyladenosine (m6A) RNA modification in macrophages and thereby regulates macrophage anti-tumor immunity.

THP-1 cells were differentiated into macrophage-like cells using phorbol 12-myristate 13-acetate (PMA) and subsequently cultured under normal methionine conditions or treated with 0.8 mM methionine for 48 h. Arraystar Human m6A-mRNA&lncRNA Epitranscriptomic Microarray analysis was performed to characterize transcriptome-wide changes in m6A modification and to identify candidate regulatory factors associated with high-methionine-induced alterations in macrophage anti-tumor immune function.