Omics · study · 2026
Comparative RNA-seq analysis of PM2.5 responses in wildtype and malignantly transformed mouse mammary epithelial cells cultured as spheroids
Listed in NCBI GEO
Exposure to fine particulate matter (PM2.5) has been implicated in cancer initiation and progression, yet its effects on normal versus transformed epithelial cells in a three-dimensional context remain incompletely understood.
Description
In this study, we performed bulk RNA sequencing of spheroid cultures of wildtype EpH4 mouse mammary epithelial cells and their malignantly transformed counterpart, EpH4-AS. Both cell types were analyzed under untreated conditions and following PM2.5 exposure.
This dataset enables comparative analysis of differential transcriptomic responses of malignant transformation and healthy WT cells after exposure to PM2.5 in a 3D culture system.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE337nnn/GSE337004 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE337004 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1483692 ncbi.nlm.nih.gov/bioproject/PRJNA1483692 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE337004 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |