Excel · study · 2026
Transcriptomic profiling of ATP6AP1 knockdown in activated human hepatic stellate LX-2 cells
Listed in NCBI GEO
Hepatic stellate cell activation is a major driver of extracellular matrix deposition during liver fibrosis.
Description
This study investigated the transcriptional changes induced by ATP6AP1 depletion in human hepatic stellate LX-2 cells. LX-2 cells were transfected with ATP6AP1-targeting siRNA or non-targeting control siRNA, with three independent biological replicates per condition.
Total RNA was extracted and subjected to RNA sequencing. Sequencing reads were aligned to the human reference genome GRCh38.p10, and differential gene-expression analysis was performed using DESeq2. The dataset was generated to identify the cellular pathways regulated by ATP6AP1 depletion, with particular emphasis on hepatic stellate cell activation, cellular senescence, cell-cycle arrest and Notch signaling
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE339nnn/GSE339298 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE339298 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1498110 ncbi.nlm.nih.gov/bioproject/PRJNA1498110 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Biochemistry and cell biology 68% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE339298 | 15 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3101 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (68%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |