Omics · study · 2026
Short-Duration HIPEC-Mimetic Exposure to Mithramycin A Induces Structured Transcriptional Reprogramming in HT-29 Colorectal Cancer Cells
Listed in NCBI GEO
This study investigates the transcriptional consequences of short-duration HIPEC-mimetic exposure to Mithramycin A (MA) in HT-29 colorectal cancer cells.
Description
Cells were exposed for 90 minutes to MA (750 nM) or Mitomycin C (4.48 µM) at 37 °C followed by drug washout. RNA sequencing was performed to determine whether transient exposure induces durable transcriptional reprogramming.
Differential expression and pathway analyses identified coordinated enrichment of cell-cycle checkpoint programs and modulation of chromatin regulatory networks. One biological replicate (MA2) was excluded due to high duplication rates and low mapping efficiency. Analyses excluding this sample are presented.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE320nnn/GSE320366 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE320366 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1427673 ncbi.nlm.nih.gov/bioproject/PRJNA1427673 ↗
project · from NCBI GEO
- PubMed 42074218 pubmed.ncbi.nlm.nih.gov/42074218 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · RNA sequencing 75% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE320366 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |