Deletion of immune regulatory factor Fgl2 affects the mouse spleen immune profile

Compared with WT group, the proportion of naive B cells, transitional B cells, marginal B cells, follicular B cells, CD4-T naive, CD8-T naive, CD4-T regulatory subsets, NKT, NK and DC subsets in Fgl2 KO group decreased. The proportion of PCs, CD4-Tem and monocyte subsets increased.

Notably, we identified four new B cell subpopulations in the Fgl2 KO group that cannot be classified as classical B cell subpopulations and are therefore named B cell_sp1, B cell_sp2, B cell_sp3, and B cell_sp4.B cell_sp1 subgroup showed dominant clones of IGHA, IGHM and IGHD in the constant region, while the other 3 different subgroups and pc only showed dominant clones of IGHA in the constant region. This suggests that IGHA clones are most abundant and dominant in Fgl2 KO B cells.