Omics · study · 2026
Hidden outcomes of CRISPR/Cas9 genome editing revealed by targeted sequencing in isogenic human iPSC clones [RNA-seq]
Listed in NCBI GEO
CRISPR/Cas9 editing can create complex on-target alterations that escape conventional PCR genotyping.
Description
We characterized ten edited human induced pluripotent stem cell clones derived from four related parental lines carrying a tandem Xq25 duplication encompassing STAG2. Targeted genomic sequencing revealed inversions, plasmid integration, and complex rearrangements and resolved discrepancies between genotyping and transcriptomic observations.
This GEO submission provides quantitative genome-browser coverage tracks supporting the transcriptomic and whole-genome analyses. BioProject: PRJNA1499378.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE341nnn/GSE341489 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE341489 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1502830 ncbi.nlm.nih.gov/bioproject/PRJNA1502830 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Agricultural biotechnology 69% · RNA sequencing 65% · Sequencing 75%
Provenance · 1 source records, 10 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE341489 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].anzsrc:group:3001 | enrichment · NCBI GEO | taxonomy-embedding@1.1.0 | title+keywords+description (69%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |