Omics · study · 2026
Thrombospondin-1, CD47, and SIRPa display cell-specific molecular signaturesin human islets and pancreata
Listed in NCBI GEO
Thrombospondin-1 (TSP1) is a secreted protein minimally expressed in health but increased in disease and age.
Description
TSP1 binds tothe cell membrane receptor CD47, which itself engages signal regulatory protein a (SIRPa), and the latter creates a checkpointfor immune activation. Individuals with cancer administered checkpoint-blocking molecules developed insulin-dependent diabe-tes.
Relevant to this, CD47 blocking antibodies and SIRPa fusion proteins are in clinical trials. We characterized the molecularsignature of TSP1, CD47, and SIRPa in human islets and pancreata. Fresh islets and pancreatic tissue from nondiabetic individu-als were obtained.
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The expression of THBS1, CD47, and SIRPA was determined using single-cell mRNA sequencing, immunofluo-rescence microscopy, Western blot, and flow cytometry. Islets were exposed to diabetes-affiliated inflammatory cytokines andchanges in protein expression were determined. CD47 mRNA was expressed in all islet cell types.
THBS1 mRNA was restrictedprimarily to endothelial and mesenchymal cells, whereas SIRPA mRNA was found mostly in macrophages. Immunofluorescencestaining showed CD47 protein expressed by b cells and present in the exocrine pancreas. TSP1 and SIRPa proteins were notseen in islets or the exocrine pancreas.
Western blot and flow cytometry confirmed immunofluorescent expression patterns.Importantly, human islets produced substantial quantities of secreted TSP1. Human pancreatic exocrine and endocrine tissueexpressed CD47, whereas fresh islets displayed cell surface CD47 and secreted TSP1 at baseline and in inflammation. Thesefindings suggest unexpected effects on islets from agents that intersect TSP1-CD47-SIRPa
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE328nnn/GSE328522 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE328522 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1455600 ncbi.nlm.nih.gov/bioproject/PRJNA1455600 ↗
project · from NCBI GEO
- PubMed 36791324 pubmed.ncbi.nlm.nih.gov/36791324 ↗
publication · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Homo sapiens
- From keywords
- Life Sciences
- Inferred from text
- Cancer 75% · Disease 75% · Microscopy 75% · Sequencing 75%
Provenance · 1 source records, 11 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE328522 | 12 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[disease].local:disease:cancer | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[disease].local:disease:disease | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:microscopy | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[modality].local:modality:sequencing | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[organism].NCBITaxon:9606 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |