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Excel · dataset · 2023

Data underlying the research of: The mRNA expression of CACYBP in bladder and kidney cancer.

Listed in DataCite

qRT-PCR Cellular RNA was extracted using Trizol, and total RNA (500 ng) was transcribed into cDNA using the PrimeScript kit.

Description

Actin primers were used as an internal control. Real-time fluorescence quantitative RT-PCR assays were performed using Lightcycler 480ii.

The qRT-PCR primer sequences used in this study are shown below: Cacybp: forward primer: 5-CTCCCATTACAACGGGCTATAC-3, reverse primer: 5-GAACTGCCTTCCACAGAGATG-3; hactin: forward primer: 5-GGCATCGTCACCAACTGGGAC-3; reverse primer: 5-CGATTTCCCGCTCGGCCGTGG-3. Obtain the Cq datas of the actin and CACYBP gene of HK-2, O-786, SV-HUC-1, T24 cells for analysis.

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Where it is published

Catalogue records · 2

Topics

Inferred from text
Cancer 75%
Provenance · 1 source records, 11 field assertions
SourceKeyLast seenRaw
DataCite10.4121/22004108.v16 d agoJSON v1
FieldAssertionExtractorEvidence
access_levelsource · DataCiteconnector:datacite@1.0.0/data/attributes/rightsList
concepts[disease].local:disease:cancerenrichment · DataCitekeyword-concept-rules@1.0.0title+description (75%)
concepts[field].fos:basic-medicinesource · DataCiteconnector:datacite@1.0.0
concepts[field].fos:health-sciencessource · DataCiteconnector:datacite@1.0.0
concepts[field].fos:medical-engineeringsource · DataCiteconnector:datacite@1.0.0
concepts[field].fos:other-medical-sciencessource · DataCiteconnector:datacite@1.0.0
descriptionsource · DataCiteconnector:datacite@1.0.0/data/attributes/descriptions
licensesource · DataCiteconnector:datacite@1.0.0/data/attributes/rightsList
publication_datesource · DataCiteconnector:datacite@1.0.0/data/attributes/dates
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