Alu repeat-containing RNA spatially organizes actively transcribed genomic regions around nuclear speckle

Highly transcribed genes and their nascent pre­mRNAs are organized around nuclear speckles, facilitating co-transcriptional RNA processing. However, the mechanism underlying this spatial organization remains unclear.

Here, we identified a class of Alu repeat-containing RNAs that are highly enriched in nuclear speckles. These RNAs interact with actively transcribed genes through their Alu elements, partially via R-loop formation. They also engage with nuclear speckle proteins promoting their aggregation, phase separation and assembly of nuclear speckles, thereby orchestrating the spatial organization of actively transcribed genes around these structures.