Data · dataset · 2021
Post-translational Modifications of ATG16L1 are essential for the stability and activation of the Autophagosome Complex
Listed in Borealis and Agri-environmental Research Data Dataverse — shown once because both records carry DOI 10.5683/sp3/xl82k5
Autophagy is a cellular stress response pathway that controls cell growth and maintains homeostasis.
Description
Dysregulation of autophagy leads to several cytopathic disorders, such as cancer, inflammatory bowel disorders, and neurodegeneration. Cellular stress, such as nutrient starvation or infection, triggers the autophagic cascade by activating the ATG16L1/5-12 protein complex, which delivers cellular compartments to lysosomes for degradation.
Very little is known about where ATG16L1 is located under nutrient rich conditions and about the first steps of its activation, prior to binding to nascent autophagosome. We discovered three post-translational modifications (PTM’s) of ATG16L1, S268A, S269/287A, and T281A, that occur under nutrient rich conditions and are lost upon starvation. We also discovered under basal conditions ATG16L1 is localized to the cytoskeleton through interaction with SPEC1L, and that ATG16L1/SPEC1L complex dissociates upon activation.
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We hypothesize that the stress-sensitive PTM’s mediate the complex’s stability; during stress, they dissociate from the ATG16L1/SPEC1L complex leading to the activation of the complex. My objective is to create phospho-mimetic and phospho-dead mutations of ATG16L1 to detect if these phosphorylation events regulate ATG16L1 inactivation at the cytoskeleton. Mutations were inserted into the genome of virus vectors by Site-Directed Mutagenesis, based on KOD Xtreme Hot Start and Pfu Turbo DNA Polymerase enzymes protocols.
Salmonella bacteria were infected with the vectors to evaluate the mutations through direct-sequencing. Mutations will be introduced in vitro into mammalian cells, and the results will be analysed by Western Blotting and Immunoprecipitation.
Links
Where it is published
- Dataverse dataset page borealisdata.ca/dataset.xhtml?persistentId=doi%3A10.5683%2FSP3%2FXL82K5 ↗
landing page · from borealisdata ca
- DOI doi.org/10.5683/sp3/xl82k5 ↗
DOI / persistent id · from borealisdata ca
Catalogue records · 1
- Dataverse API borealisdata.ca/api/datasets/:persistentId/?persistentId=doi%3A10.5683%2FSP3%2… ↗
metadata API · from borealisdata ca
Topics
- Stated by source
- Medicine, Health and Life Sciences · Medicine, Health and Life Sciences
- From keywords
- Chemistry · Computer Science & AI · Earth & Environmental Science · Economics & Finance · Engineering · Humanities · Life Sciences · Life Sciences · Mathematics & Statistics · Medicine & Health · Medicine & Health · Ocean & Atmospheric Science · Social Science
- Inferred from text
- Cancer 75% · Oncology and carcinogenesis 69%
Provenance · 2 source records, 23 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| Borealis | doi:10.5683/SP3/XL82K5 | 12 d ago | JSON v1 |
| Agri-environmental Research Data Dataverse | doi:10.5683/SP3/XL82K5 | 11 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| concepts[disease].local:disease:cancer | enrichment · borealisdata ca | keyword-concept-rules@1.0.0 | title+description (75%) |
| concepts[field].anzsrc:group:3211 | enrichment · borealisdata ca | taxonomy-embedding@1.0.0 | title+keywords+description (69%) |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].dataverse_subject:medicine-health-and-life-sciences | source · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:chemistry | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:computer-science-ai | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:earth-environmental | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:economics-finance | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:engineering | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:humanities | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:life-sciences | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:mathematics-statistics | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:medicine-health | mapping · borealisdata ca | connector:borealisdata_ca@1.0.0 | /subjects |
| concepts[field].local:field:ocean-atmospheric | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| concepts[field].local:field:social-science | mapping · borealisdata ca dataverse | connector:borealisdata_ca_dataverse@1.0.0 | /subjects |
| created_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| description | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /description |
| publication_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| title | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | /name |
| updated_date | source · borealisdata ca | connector:borealisdata_ca@1.0.0 | |
| version_label | source · borealisdata ca | connector:borealisdata_ca@1.0.0 |