Excel · study · 2026
Consequences of reactive astrogliosis in vivo I
Listed in NCBI GEO
To investigate how molecularly induced reactive astrogliosis (MiRA) affects the transcriptomic signatures of astrocytes in the striatum, we used locally injected AAV2/5 GfaABC1D-RiboTag to purify and sequence astrocyte actively translated mRNAs in the striatum of the control and MiRA animals.
Description
Three weeks after AAV injection, striata were homogenized, and RNA was purified from (i) cleared lysate as the input and control, and (ii) astrocyte-specific ribosome-associated RNA precipitated via a hemagglutinin (HA) tag.
Our analyses revealed that MiRA induces inflammatory signatures while downregulating the homeostatic genes in the striatal astrocytes.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE305nnn/GSE305388 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE305388 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1305448 ncbi.nlm.nih.gov/bioproject/PRJNA1305448 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
Provenance · 1 source records, 7 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE305388 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |