Omics · study · 2026
RNA-seq analysis of Tada2b knockout in B16F10 cells
Listed in NCBI GEO
Tada2b (Transcriptional adaptor 2B) is a core component of the SAGA complex, where it serves as a critical adaptor for the histone acetyltransferase Gcn5.
Description
While Tada2b is known to facilitate gene activation, its specific role in melanoma and the maintenance of the transcriptome remains poorly defined. In this study, we investigated the global gene expression changes resulting from Tada2b loss in B16F10 murine melanoma cells.
We generated Tada2b-knockout (KO) cells using CRISPR/Cas9 and performed bulk RNA-sequencing. Our goal was to identify differentially expressed genes and dysregulated pathways that depend on Tada2b-mediated transcriptional regulation.
Links
Get the data
- GEO FTP directory ftp.ncbi.nlm.nih.gov/geo/series/GSE318nnn/GSE318799 ↗
download · from NCBI GEO
Where it is published
- GEO accession page ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE318799 ↗
landing page · from NCBI GEO
Documentation and papers
- PRJNA1420645 ncbi.nlm.nih.gov/bioproject/PRJNA1420645 ↗
project · from NCBI GEO
Topics
- Stated by source
- Expression profiling by high throughput sequencing · Mus musculus
- From keywords
- Life Sciences
- Inferred from text
- RNA sequencing 65%
Provenance · 1 source records, 8 field assertions
| Source | Key | Last seen | Raw |
|---|---|---|---|
| NCBI GEO | GSE318799 | 14 d ago | JSON v1 |
| Field | Assertion | Extractor | Evidence |
|---|---|---|---|
| access_level | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[field].local:field:life-sciences | mapping · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| concepts[method].geo_series_type:expression-profiling-by-high-throughput-sequencing | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /gdstype |
| concepts[modality].local:modality:rna-seq | enrichment · NCBI GEO | keyword-concept-rules@1.0.0 | title+description (65%) |
| concepts[organism].NCBITaxon:10090 | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /taxon |
| description | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /summary |
| publication_date | source · NCBI GEO | connector:ncbi_geo@1.0.0 | |
| title | source · NCBI GEO | connector:ncbi_geo@1.0.0 | /title |